Travelling
Hi, I’m
DANIELA NATALE
Principal Research Scientist
ROMA
Summary
Challenges lover and results-oriented scientist with 9+ years experience in a Contract Research Organization. Extensive hands-on experience in cell biology and immuno-oncology. Highly adaptable relationship builder and excellent communicator, fast learner, willingness to seek new learning opportunities and responsabilities.
Overview
13
years of professional experience
10
years of post-secondary education
3
Languages
Work History
IRBM - Oncology UnitPomezia
Principal Research Scientist
03.2022 - Current
Job overview
- Support drug discovery programs by providing relevant scientific and strategic input by designing and executing relevant immune assays and immuno-profiling experiments for Immuno-Oncology projects
- Characterization of tumor micro-environments with multi-color flow cytometry
- Streamlined research processes to meet tight deadlines for multiple projects
- Wrote research papers, reports and summaries regarding projects in immunology and oncology
- Expanded cross-functional organizational capacity by collaborating across departments on priorities, functions and common goals
- Performed research into study topics to increase knowledge and to provide valuable contributions
- Responsible of the Flow Cytometry and Cell Sorting Platform: oversee all aspects of method development, troubleshooting, validation and sample analysis for research studies requiring flow cytometry
- Documented and reported test results after making graphical solutions and representations.
- Developed and maintained relationships with customers and suppliers
- Provided detailed technical and operational direction in project challenges, consistently meeting deliverables according to deadlines
- Made decisions independently and collaboratively on projects-related challenges
IRBM S- Discovery Biology Department
Principal Research Scientist
01.2019 - 03.2022
Job overview
- Design and perform flow cytometry and cell based experiments to support a bespoke assay service in human and mouse immunology, including immune-oncology
- Provide training and technical support to researchers across IRBM teams for FACS-based cell isolation and characterization associated with in vitro and in vivo studies
- Support scientists with presentations/reports/data analysis for all flow cytometry-based studies
- Maintaining and trouble-shooting cutting-edge equipment (FACS Canto II, FACS Aria III, Elispot)
- Performed research activities for Antibodies Screening programs in High-Throughput mode (HTS)
- Collaborated with leadership team to identify relevant questions and determine best methods of collection
- Generated data models and performed analysis to produce reports outlining results
IRBM - Translational research DepartmentRome
Research Scientist
01.2013 - 01.2018
Job overview
- Support small molecules screenings by providing scientific input, conduct and execute research activities for assay target selection, design and validation
- Manage a scientific team of 2-3 FTE’s (senior scientists and research associates)
- Oversaw and developed different research projects
- Writing manuscripts for scientific publication, project reports and conference presentations
- Collaborated with leadership team to identify relevant questions and determine best methods of collection
- support the establishment of FACS facility
- Responsible for maintaining cutting-edge equipments, provide training and technical support to researchers
- Perform multi-colour flow cytometric cell sorting and FACS experiments
- Analyze and interpretate experimental data by using of FACS analysis softwares (FlowJo, Modfit, FCS Express)
I.I.S. Cartesio Luxemburg
Chemistry Teacher
10.2012 - 10.2012
Job overview
- Evaluated and revised lesson plans and course content to achieve student-centered learning.
- Participated in department meetings to provide input to colleagues about student achievement and improvement.
- Prepared plans, materials and activities for students to reach chemistry learning outcomes.
Catholic University of Rome, Bambino Gesù Children’s HospitalRome
AIRC Postdoctoral Fellow
04.2010 - 09.2012
Job overview
- Investigation of the mechanism underlying immunosurveillance escape of cancer and the related role of the enzyme indoleamine 2,3 dioxygenase (IDO) in adult and pediatric patients with hematological malignancies
- Isolation of peripheral blood mononuclear cells (PBMCs) using Ficoll
- Generation of dendritic cells from isolated monocytes
- Analysis of cell populations by Flow Cytometry (BD FACS Canto II) in biological samples (blood and bone marrow from pediatric patients)
- Preparation of biological samples for High Performance Liquid Cromatography
- Western Blots and immunofluorescence
- Learned new skills and applied to daily tasks to improve efficiency and productivity
Theodor Kocher Institute - University of BernBern
Marie Curie Postdoctoral Fellowship
01.2008 - 02.2010
Job overview
- In vivo analysis of lymphocytes migration by combining quantum dot technology and twophoton microscopy
- Synthesis of antibody-nanoparticle conjugates
- Agarose gel electrophoresis for antibody purification
- Cleaning of mouse lymph nodes and preparation of their cryosections
- Cells isolations from mice
- Immunofluorescence of cells and tissue sections, fluorescence microscopy and in vivo multiphoton imaging
- Video processing and analysis (softwares: ImageJ and Volocity)
- Identified issues, analyzed information and provided solutions to problems.
- Collaborated with team members to achieve target results.
Education
University of Edinburgh
PhD
09.2004 - 12.2008
School of Chemistry, University of Naples Federico II
Master of Science
Degree from Chemistry
09.1997 - 10.2003
Skills
Critical thinking
Excellent oral/written communication skills
Artistic aptitudeExperiment Design
Training and mentoring
Report generation
Data analysis
Collaborative
Work well under pressure
Results orientated
Interests
Photography
Shiatsu (IES student)
Meditation
Artistic activities
Timeline
Principal Research Scientist
IRBM - Oncology Unit
03.2022 - Current
Principal Research Scientist
IRBM S- Discovery Biology Department
01.2019 - 03.2022
Research Scientist
IRBM - Translational research Department
01.2013 - 01.2018
Chemistry Teacher
I.I.S. Cartesio Luxemburg
10.2012 - 10.2012
AIRC Postdoctoral Fellow
Catholic University of Rome, Bambino Gesù Children’s Hospital
04.2010 - 09.2012
Marie Curie Postdoctoral Fellowship
Theodor Kocher Institute - University of Bern
01.2008 - 02.2010
University of Edinburgh
PhD
09.2004 - 12.2008
Degree from Chemistry
09.1997 - 10.2003
School of Chemistry, University of Naples Federico II
Master of Science