Elena Caproni

Medical Biotechnologist, with a strong Cellular/Molecular Biological and Microbiological background focused on Vaccine research. 5-years work experience at Novartis Vaccines and Diagnostics Research Center as Research Associate, following a brief experience in Manufacturing Science and technology Department. Currently employed as Pre-clinical Researcher at Toscana Life Sciences and involved in a project on tumor immunotherapy. Analytical and synthetic spirit, ability to identify critical issues in complex projects. Highly motivated, result driven, able to run a project autonomously but with great ability to work in a team. Excellent presentation and communication skills.

Cell Biology Skills: Cell culture and manipulation procedures for human/mouse cell lines, primary cells isolation (from mice or human samples) and culture, transfection, cell-based assays to investigate innate immune receptor activation, MAT (Monocyte-Activation Test), LDH cytotoxicity assay, FACS analysis of cell/bacteria surface markers., Molecular Biology Skills: protein purification from cells, western Blot assay, RNA purification from cells and tissues, Microarray-based gene expression analysis, silencing, RT and Q-PCR analysis., Bacteriology Skills: manipulation of pathogens in BSL2 laboratory, measurement of bacterial association and invasion by viable counting, purification and characterization of OMVs from different E.Coli mutants., Diagnostic assays: ELISA assay, ELISpot assay, MesoScale assay., Microscopy skills: staining of tissue-cryosections and confocal microscopy analysis, staining of cells/bacteria and confocal microscopy analysis., Knowledge of GLPs and GMPs., Italian, Italian, English, French, Windows XP and Mac OS X operating systems., Office Pack, DNA-proteins analysis softwares (FlowJo, BASE, TMeV, IPA, GeneSpring), imaging softwares (LSM Image Browser)., Travel, Music, Theater, Opera, Dance, Running, Gym, Swim, Triathlon and sports in general., Very interested in Nutrition and in particular Sport Nutrition.

I hereby grant permission to use my personal data in accordance with the italian Leg. Dec. 196/2003

10/08/1982

+393497590224

• 01/2008, 04/2011, PhD in Cellular Biology, University of Padua, Padua, Italy
• 10/2005, 07/2007, Master Degree in Medical Biotechnology, University of Florence, Florence, Italy
• 09/2002, 09/2005, Diploma degree in Biotechnology, University of Florence, Florence, Italy
• July 2001, High school diploma, Gymnasium liceum “A. Mariotti”, Perugia, Italy

Nationality: Italian

• Finco O., Frigimelica E., Buricchi F., Petracca R., Meoni E., Galli G., Faenzi E., Bonci A., Agnusdei M., Nardelli F., Bartolini E., Scarselli M., Caproni E., Zedda L., Skibinski D., Giovinazzi S., Bastone R., Ianni E., Cevenini R., Grandi G., and Grifantini R.. Approach to discover T and B cell antigens of intracellular pathogens applied to the design of Chlamydia trachomatis vaccines (Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):9969-74. Epub 2011 May 31).
• Montanari P., Bozza G., Capecchi B., Caproni E., Barrile R., Norais N., Capitani M., Sallese M., Cecchini P., Ciucchi L., Gao Z., Rappuoli R., Pizza M., Aricò B. and Merola M. Human heat shock protein Hsp90 interferes with Neisseria meningitidis adhesin A (NadA)-mediated adhesion and invasion. (Cell Microbiol. 2011 Nov 8. doi: 10.1111/j.1462-5822.2011.01722.x.).
• Caproni E., Tritto E., Cortese M., Muzzi A., Mosca M., Monaci E., Baudner B., Seubert A. and De Gregorio E. MF59 and Pam3CSK4 boost adaptive responses to influenza subunit vaccine through an IFN type I independent mechanism of action. (J. Immunol 2012; 188:3088:3098 February 2012)
• De Gregorio E., Caproni E., Ulmer J.B. Vaccine Adjuvants: mode of action. (Front Immunol. 2013 Jul 31;4:214. doi: 10.3389/fimmu.2013.00214. eCollection 2013).
• Bozza G, Capitani M, Montanari P, Benucci B, Biancucci M, Nardi-Dei V, Caproni E, Barrile R, Picciani B, Savino S, Aricò B, Rappuoli R, Pizza M, Luini A, Sallese M, Merola M. Role of ARF6, Rab11 and External Hsp90 in the Trafficking and Recycling of Recombinant-Soluble Neisseria meningitidisAdhesin A (rNadA) in Human Epithelial Cells. (PLoS One. 2014 Oct 27;9(10):e110047. doi: 10.1371/journal.pone.0110047.)
• Wu TY, Singh M, Miller AT, De Gregorio E, Doro F, D'Oro U, Skibinski DA, Mbow ML, Bufali S, Herman AE, Cortez A, Li Y, Nayak BP, Tritto E, Filippi CM, Otten GR, Brito LA, Monaci E, Li C, Aprea S, Valentini S, Calabrό S, Laera D, Brunelli B, Caproni E, Malyala P, Panchal RG, Warren TK, Bavari S, O'Hagan DT, Cooke MP, Valiante NM. Rational design of small molecules as vaccine adjuvants. Sci Transl Med. 2014 Nov 19;6(263):263ra160.
• Zerbini F, Zanella I, Fraccascia D, König E, Irene C, Frattini L F, Tomasi M, Fantappiè L, Ganfini L, Caproni E, Parri M, Grandi A and Grandi G. Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli. Microbial cell Factory. 2017 Apr 24;16(1):68. doi: 10.1186/s12934-017-0681-1.
• Grandi A, Tomasi M, Zanella I, Ganfini L, Caproni E, Fantappiè L, Irene C, Frattini L, Isaac SJ, König E, Zerbini F, Tavarini S, Sammicheli C, Giusti F, Ferlenghi I, Parri M, Grandi G. Synergistic Protective Activity of Tumor-Specific Epitopes Engineered in Bacterial Outer Membrane Vesicles. Frontiers in Oncology 2017 Nov 7;7:253. doi: 10.3389/fonc.2017.00253. eCollection 2017.
• Tomasi M, Dalsass M, Beghini F, Zanella I, Caproni E, Fantappiè L, Gagliardi A, Irene C, König E, Frattini L, Masetti G, Isaac SJ, Armanini F, Cumbo F, Blanco-Míguez A, Grandi A, Segata N, Grandi G. Commensal Bifidobacterium Strains Enhance the Efficacy of Neo-Epitope Based Cancer Vaccines. Vaccines (Basel). 2021 Nov 18;9(11):1356. doi: 10.3390/vaccines9111356.
• Tomasi M, Caproni E, Benedet M, Zanella I, Giorgetta S, Dalsass M, König E, Gagliardi A, Fantappiè L, Berti A, Tamburini S, Croia L, Di Lascio G, Bellini E, Valensin S, Licata G, Sebastiani G, Dotta F, Armanini F, Cumbo F, Asnicar F, Blanco-Míguez A, Ruggiero E, Segata N, Grandi G, Grandi A. Outer Membrane Vesicles From The Gut Microbiome Contribute to Tumor Immunity by Eliciting Cross-Reactive T Cells. Front Oncol. 2022 Jun 30;12:912639. doi: 10.3389/fonc.2022.912639.