Websites
Summary
Accomplishments
Skills
Work History
Publications
Projects
Education
Certification
Overview
Work Availability
Interests
Affiliations
Timeline
Languages
Hobbies
web
Farhan Ali, PhD

Farhan Ali, PhD

Research Scholar
Rome,RM

Summary

Molecular Plant Biologist (PhD) with 5+ years’ R&D experience in microbial biocontrol, molecular diagnostics, and applied genomics. Proven record of developing scalable lab workflows (phenotypic, NGS, SEM, EPS quantification) and leading cross-functional teams to reduce costs and accelerate product development. Skilled in assay design, data analysis (R, GraphPad, IGV), GMP-compliant and QA/QC workflows (development, validation, monitoring), and translating research into operational solutions for agri-biotech and food-safety applications.

Accomplishments

PhD Research - University of Molise (2020-2025)

  • Reduced reagent costs 60% via pooled NGS and multiplex bioassays
  • Generated ~700 AMT transformants; mapped EPS gene insertions (NGS/IGV)
  • Trained 4 interns on lab workflows, NGS analysis, and equipment operation

M.Sc (Hons) Research - CEMB (2018-2019)

  • Streamlined bacterial biocontrol strain preservation (saved time/resources)
  • Identified synergistic BCA-Alternaria solani interactions

B.Sc (Hons) Research - Citrus Research Institute (2017)

  • Identified top-performing tomato variety (Sahel) via disease analysis
  • Coordinated field management: 20% yield increase (45t/acre)

Skills

Molecular biology

  • AMT: Plasmid design, transformation, colony screening
  • DNA/RNA Extraction
  • PCR
  • Sampling and data collection
  • Mass culturing

Genomics & bioinformatics tools

  • SnapGene
  • IGV (Integrative Genomics Viewer)
  • NGS data analysis tools
  • Experimental design
  • NCBI tools (Genbank, BLAST)

Microscopy & imaging

  • Histopathological Analysis
  • Scanning-Electron Microscopy
  • ImageJ
  • Compound microscopy
  • Stereo microscopy

Assays & phenotyping

  • In Vivo and In Vitro experiments
  • EPS quantification
  • Tecan i-control
  • UV Spectrophotometry

Data & statistics

  • R Programming
  • GraphPad PRISM
  • ClustVis web tool
  • Scientific Writing
  • Microsoft 365

Laboratory management

  • Training and supervision
  • Inventory management SOPs

Team Leadership

  • Trained four interns in laboratory workflows, NGS data analysis, and equipment operation at the University of Molise (2022–2025)

Research Project Coordination

  • Coordinated experimental workflows, data collection, and reporting during a multi-year PhD research project (Prot: 2022TPZW3)

Laboratory Resource Management

  • Managed laboratory inventories, experimental resources, and equipment scheduling for the research team

Scientific Communication

  • Conferences, posters, and oral presentations (EuroDisBioFood Workshop, UNIMOL PhD Expo, EcoSET PhD Conference, ECGF16)

Work History

Research Scholar

University of Molise
Campobasso, Italy
11.2020 - 09.2025

• Investigated the molecular mechanisms underlying biocontrol activity in the yeast Papiliotrema terrestris against Penicillium expansum
• Designed and carried out exopolysaccharide (EPS) assays and antagonism experiments using in vitro and in vivo approaches.
• Generated ~700 transformants using Agrobacterium-mediated transformation and identified EPS-impaired mutants.
• Mapped T-DNA insertion sites using next-generation sequencing and Integrative Genomics Viewer (IGV).
• Supervised laboratory interns and coordinated experimental workflows and laboratory resource management.
• Presented research findings at international conferences and authored project reports, abstracts, and peer-
reviewed manuscripts.

Research Intern

Centre of Excellence in Molecular Biology
Lahore, Pakistan
12.2018 - 08.2019

• Conducted molecular characterization of bacterial and fungal isolates using DNA extraction, PCR, and sequencing.
• Performed phylogenetic analyses to evaluate genetic relationships among microbial strains.

• Supported laboratory workflows, including media preparation and experimental setup.

• The interaction between BCAs and A. solani has led to new insights into the synergy among different microbes. These findings could lead to the development of combined microbial products in the future.

Intern

Citrus Research Institute
Sargodha, Pakistan
01.2017 - 06.2017

• Analyzed disease severity and incidence data in tomatoes (Sahel variety), identifying the area's most outperforming and high-yielding variety.
• Analyzed disease severity and incidence data in Sahel variety tomatoes and identified the area's most outperforming and high-yielding variety.
• Applied technical knowledge in soil analysis, irrigation planning, and crop monitoring to coordinate team efforts and enhance field management, resulting in an approximate 20% yield increase (45t/acre) over the previous two years.

Retail Sales Specialist

Khawaja Fabric
Sargodha, Pakistan
10.2013 - 12.2016

• Increased sales by 25% and improved customer satisfaction through personalized servicing.
• Provided in-depth product information, resulting in a 40% increase in educated customer purchases and strong customer relationships.
• Leveraged strong communication skills to build customer rapport, fostering long-term connections and contributing to a 35% growth in sustained business.

Retail Sales Specialist

New Khawaja Medicine
Sargodha, Pakistan
08.2012 - 08.2013

• Managed efficient cash operations and prioritized helping customers over completing other routine tasks in-store.
• Developed a strong rapport with customers and created a positive impression of the business.
• Opened, shelved, and merchandised new products in visually appealing and organized displays for optimal sales promotions.
• Maintained up-to-date knowledge of store sales, payment policies for distributors, and security standards regarding expiry and critical drugs.

Publications

2023

Title: Mycotoxin production by Fusarium species and a recent deep insight into management through biocontrol approaches

Peer-reviewed research article
Authors: Amjad Ali, Fatih Ölmez, Muhammed Tatar, Irum Tabbasum, Hajra Azeem, Rabia Sarwar, Farhan Ali | 

Journal Name:Journal of Global Innovations in Agricultural Sciences  

Volume, Issue and Pages: 11, 455–480 | Publisher: GIAS Publishers

2023

Title: Title: Efficacy of Azadirachta indica and Datura stramonium Extract with Ethanol against Different Life Stages of Tribolium castaneum

Peer-reviewed research article
Authors: Naeem, H., Ali, F., Sarfaraz, S., Sharif, U., Hussain, S. G., Ameer, F., ... & Kanwal, H. | 

Journal Name: Asian Journal ofAdvanced Agricultural Research | 

Volume, Issue and Pages: 23(1), 59–65 | Publisher: Science Domain International

2023

Title: Review on Biological Management of Bactrocera zonata through Pathogenic Activity of Beauveria bassiana

Peer-reviewed research article

Authors: Ali, H. M. S., Naqvi, S. H. K., Ullah, M. Z., Ali, F., Sarfaraz, S., Fawaz, M. S. Mushtaq, I. | 

Journal Name: Asian Journal of Research in Crop Science

Volume, Issue and Pages: 8(4), 543–550 | Publisher: Science Domain International

2023

Title: Phytoremediation of copper and arsenic contaminated soil using Gladiolus grandiflorus and Chrysanthemum morifolium plants

Peer-reviewed research article
Authors: Amjad, N., Akhtar, I., Hameed, A., Ahmad, F., Ali, F., Ahmad, M., & Ahmad, U. I.  

Journal Name: Asian Journal of Soil Science and Plant Nutrition 

Volume, Issue and Pages: 9(2), 36–42 | Publisher: Science Domain International

2023

Title: Extraction and Characterization of Cuticle Degrading Enzymes of Beauveria bassiana for Enhanced Pathogenicity against Bactrocera dorsalis

Peer-reviewed research article

Authors: Ullah, S., Naeem, H., Murtaza, A., Sharif, U., Sarfaraz, S., Ali, F., Sumbal, G. A. K., Ullah, U., & Kanwal, M.

Journal Name: Asian Journal of Advances in Agricultural Research

Volume, Issue and Pages: 23(2), 225–235 | Publisher: Science Domain International

2022

Title: Isolation, Characterization and Management of Alternaria solani causing Early Blight in Tomato through different Bacterial strains

Peer-reviewed research article
Authors: Subhani, M. N., Ali, F., Nasir, S., & Shahid, A. A. 

Journal Name: Plant Cell Biotechnology and Molecular Biology

Volume, Issue and Pages: 23(31–32), 78–88 | Publisher: International Knowledge Press

2023

Title: AMT-mediated insertional mutagenesis in Papiliotrema terrestris PT22AV for assessing the role of extracellular polysaccharide (biofilm) production in biocontrol activity

Authors: F. Ali, R. Castoria, D. Palmieri, G. Ianiri | 

Publisher: EcoSET International PhD Conference, University of Molise

Abstract included in Book of Abstracts, EcoSET International PhD Conference (6/6/2023). 

Available on request from the University of Molise archives.

2022

Title: AMT-mediated insertional mutagenesis in Papiliotrema terrestris PT22AV for assessing the role of extracellular polysaccharide (biofilm) production in biocontrol activity

Authors: Farhan Ali, Raffaello Castoria, Giuseppe Ianiri | 

Publisher: UNIMOL PhD Expo, 1st Edition, University of Molise

Abstract published in the E-book of Abstracts, UNIMOL PhD Expo 2022. Available on request from the University of Molise archives.

Projects

01/11/2020 – 31/01/2024

AMT-mediated insertional mutagenesis in the biocontrol agent Papiliotrema terrestris PT22AV for assessing the role of Exopolysaccharides (EPS) production in biocontrol activity, University of Molise, Italy

This project (Prot: 2022TPZW3) aims to investigate the role of exopolysaccharides (EPS) in the biocontrol yeast Papiliotrema terrestris strain PT22AV and identify genes involved in EPS production. Funded by Italian National Recovery and Resilience Plan (PNRR)

30/11/2018 – 14/09/2019

Molecular Characterization of Alternaria solani and its Biological Management, University of the Punjab, Pakistan

This project focused on the molecular characterization of Alternaria solani and its biological management through the application of plant growth-promoting rhizobacteria (PGPRs) under both in vitro and in vivo conditions. Phylogenetic analysis was conducted at the Center of Excellence in Molecular Biology to assess genetic homology.

09/02/2017 – 09/08/2017

Effect of ToMV on physiology of tomato plants & its management, Citrus Research Institute, Pakistan

The objective of this project is to evaluate and compare the efficacy of several eco-friendly chemicals—milk, eggplant extract, NPK solution, and boron solution—in controlling Tomato Mosaic Virus (ToMV). Additionally, the study examines the incidence and severity of ToMV on the Sahal tomato variety.

Education

Ph.D. - Plant Pathology

University of Molise
Campobasso, Italy
11.2020 - 09.2025

Master of Science - Plant Pathology

University of The Punjab
Lahore, Pakistan
10.2017 - 11.2019

Bachelor of Science - Plant Pathology

University of Sargodha
Sargodha, Pakistan
09.2013 - 08.2017

Certification

[The Energy for Growth (Erasmus + Youth Exchange)], [ Academy of Growth e.V., Germany] - [21/08/23-01/09/23]

The ten-day project facilitated participants' engagement with sustainable agriculture, organic food practices, and permaculture.

Mode of learning: Project-based
Link: https://www.youthpass.eu/en/verify/XSYZ-LWVM-ESDC-8T8R/Farhan Ali

[PRAXIS (Erasmus + Youth Exchange], [ Sustech Ability (SuA), Portugal] - [07/10/24-15/10/24]

Youth exchange focused on democracy, communication, and inclusion, utilizing debates, collaborative teamwork, and digital platforms.

Mode of learning: Project-based

Overview

5
5
years of professional experience
4
4
Languages
11
11
years of post-secondary education

Work Availability

monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
swipe to browse

Interests

Research and development

Sciences and innovation

Investing and Entrepreneurship

IT and machine learning

Affiliations

  • University of Sargodha, Sargodha, Pakistan.
  • Centre for Excellence in Molecular Biology, Lahore, Pakistan.
  • University of the Punjab, Lahore, Pakistan.
  • University of Molise, Campobasso, Italy.
  • Citrus Research institute, Sargodha, Pakistan.
  • AgroVentures, EU.

Timeline

Ph.D. - Plant Pathology

University of Molise
11.2020 - 09.2025

Research Scholar

University of Molise
11.2020 - 09.2025

Research Intern

Centre of Excellence in Molecular Biology
12.2018 - 08.2019

Master of Science - Plant Pathology

University of The Punjab
10.2017 - 11.2019

Intern

Citrus Research Institute
01.2017 - 06.2017

Retail Sales Specialist

Khawaja Fabric
10.2013 - 12.2016

Bachelor of Science - Plant Pathology

University of Sargodha
09.2013 - 08.2017

Retail Sales Specialist

New Khawaja Medicine
08.2012 - 08.2013

Languages

English
Bilingual or Proficient (C2)
Italian
Elementary (A2)
Urdu
Bilingual or Proficient (C2)
Punjabi
Bilingual or Proficient (C2)

Hobbies

Book reading, swimming, cycling, jogging, travelling, movies, cooking, political documentaries. 

Farhan Ali, PhDResearch Scholar