

I am currently pursuing a PhD in the MRM (Molecular and Regenerative Medicine) program at the University of Modena and Reggio Emilia, conducting my research in the laboratory of Prof. Michele De Luca under the co-supervision of Prof. Elena Enzo. My research focuses on investigating the role of irradiated mouse fibroblasts used as a feeder layer for the in vitro expansion of primary keratinocytes intended for clinical skin graft production. The goal of this project is to develop a fibroblast-free culture system that replicates the biological and mechanical properties of the current clinical-grade feeder layer while simplifying the manufacturing process, reducing production costs, and addressing regulatory challenges associated with epidermal graft production. Part of our results are currently in press in Nature Communications. End date: 31st October 2026. Estimated defense date: Beginning of April 2027.
Cell culture (primary, immortalized, cancer cells)
Feeder Layer preparation
Colony Forming Efficiency analysis
Clonal analysis
IF
IHC
WB
Confocal/fluorescence/optical microscopy
Image analysis
PCR-qPCR-ddPCR
CRISPR Cas9-based nucleofection
scRNA-seq
Scratch assay
RNA/DNA/protein extraction
Flow cytometry basics
Microsoft Office package
Prism 9
Critical data analysis
Ability to work in a multidisciplinary research team
Problem setting and problem solving
Ability to make decisions and take responsibility
Willingness to relate
Ability to listen and learn
Paper/review writing
Sense of initiative
Flexibility and adaptability
Planning and organizing the laboratory schedule
Volunteer educator of children aged 6 to 13 years old at Summer camps
"A stretch-responsive fibroblast program promotes epidermal stem cell self-renewal during skin expansion" (Article in-press).
Gaia Andrea Gozza, Caroline Aguilera Stewart, Ceyhun Alar, Leslie Bargsted, Ioanna Kaklamanou, Maria Pia Polito, Giulia Bergamini, Lorenzo Tagliazucchi, Andrea Alessandrini, Maria Paola Costi, Elena Enzo, Alejandro Sifrim & Mariaelceste Aragona. (Nature Communications,2026) https://doi.org/10.1038/s41467-026-73979-0.
"Biochemical characterization of the feedforward loop between CDK1 and FOXM1 in epidermal stem cells"
Maria Pia Polito, Alessio Romaldini, Lorenzo Tagliazucchi, Grazia Marini, Federica Radice, Gaia Andrea Gozza, Giulia Bergamini, Maria Paola Costi & Elena Enzo. (Biology Direct, 2024) https://doi.org/10.1186/s13062-024-00540-8. Volume 19, article number 91 (2024).