Ilaria Barone
Milano
Summary
Innovative and motivated Neuroscientist with >15 years of experience in understanding synaptic molecular mechanisms in vivo and in vitro to target neurological diseases.
Strong background in electrophysiology, imaging, cell culture and biochemistry: hands on in vitro, in vivo immunoprecipitation and western blotting for protein-protein interaction.
Design, execution, and lead of research projects independently
and in collaboration with heterogeneous groups of professionals.
Highly motivated scientist with ability to multitask, prioritize work
and effectively communicate data and ideas to team members.
Passionate and effective teacher which love to combines enthusiasm and dedication to create a positive and engaging learning environment for students.
Overview
16
16
years of professional experience
Work History
Principal Investigator-Senior Scientist
Hemera srl-University of Milan and Verona Spin-off
Milan
01.2023 - Current
- Lead and supervise the therapy development group.
- Responsible for overseeing the development, validation, and supply of cell-based and genetic experimental therapeutic products for early phase clinical trials.
- Design supervision and execution of antibodies optimization by Flow Cytometry, ELISA, immunofluorescence for Human derived macrophages, human derived neuronal-like cell line (SHSY-5Y), human meninges and cortex.
- Design supervision and execution of dose-response in vitro assay by gene expression interference shRNA, siRNA on human derived macrophages, human derived neuronal-like cell line, mouse primary neurons.
- Responsible for financial management, human resource management of therapy development group.
- Autonomously craft SOPs and workflows with proper documentation.
- Responsible for developing and adhering to SOPs and workflows with proper documentation.
- Participate in Technology Transfer from process development to manufacturing.
- Responsible to craft GMP documents for representative authority.
- Examine data and preparing technical reports, summaries, protocols, and quantitative analysis and ensuring experiments are well documented for submission to dedicated authority.
Associated Scientist
Boston Children's Hospital
Boston
08.2024 - Current
- Managed multiple projects simultaneously, demonstrating exceptional time management skills while maintaining high levels of accuracy.
- Led training sessions on lab equipment usage for new hires, promoting a culture of continuous learning within the team.
- Mentored junior scientists, providing guidance on experimental design and data analysis.
- Established effective collaborations with external partners to leverage resources and expertise for project success.
Adjunct Faculty Part Time
Eastern Virginia Medical School- Department of Reproductive Clinical Science
05.2022 - 04.2023
- Developed and taught educational courses in the RCS curriculum.
- Designed and implemented measures to assess the effectiveness of students’ learning in the form of various methods of learning assessments (i.e., written and oral exams) and timely grading of these assessments.
- Engaged in discussion board activities related to the courses taught.
- Advised students who need additional educational support to achieve desired learning objectives.
- Mentored students with alternative needs for their research projects as well as seminar and journal club activities.
Postdoctoral Research Fellow in Lipton Lab
Harvard Medical School - Boston Children’s Hospital, USA
01.2017 - 01.2023
- First Post doc joining the laboratory demonstrate an high degree of problem solving and organizational ability.
- Established a molecular mechanism that links sleep disorders and synaptic abnormalities by using in vivo and in vitro techniques.
- Extensive hands-on experience in design and execute experimental strategies to validate molecular targets by exploring pathways and establishing biomarkers by biochemistry: in vitro, in vivo immunoprecipitation, Kinase assay, wester blot, preparation of tissue/cells for proteomics analysis (peptide count, TMT labelling, phosphor-proteomic).
- Hands on in: Primary cell culture (neurons) and cell lines (Hela, MEFs). In vitro Electrophysiology recording by micro-electrode array (MEA), Calcium imaging recordings, patch clamp recordings, immunofluorescence-based and staining preparation of the samples in miniaturized formats (96 wells), immunohistochemistry, a wide array of molecular and cellular biological techniques, shRNA, RNA expression profiling and data analysis.
- Deep understanding of image analysis and data analysis software (ImageJ/Fiji) Cell profile, GraphPad.
- Awarded of 150.000 $ from TS Alliance in 2019.
Teaching Fellow for the GenED course 1038 Sleep
Harvard Medical School - Harvard teaching hospital
01.2020 - 01.2023
- Ranked as the 10th best TF for the fall 2021 and 2022 (response ratio 85%).
- Created and taught rigorous lessons to students.
- Create and design dedicated lessons to student with special needs.
- Learned strategies and completed workshops with a focus on classroom management (“scientist teach science”-Harvard University. “how to manage an inclusive teaching” Harvard University).
- Confidently acquired the necessary skills to facilitate a stable and inclusive learning environment.
- Worked in a 12:1:1 classroom setting and tailored specific lessons and tests.
Postdoc in the Margherita Maffei lab
Scuola Normale Superiore, Pisa, Italy
Pisa
01.2015 - 01.2016
- Conceived, led, and successfully completed several research projects for which I developed assay and rodent models for neurological disorders (obesity, depression) with the goal of identifying molecular mechanisms of pathogenesis by patch clamp recordings to enable novel treatment options using cutting edge experimental technologies.
- Identification of the pathogenetic mechanisms underlying mTOR pathway modification, a hallmark of obesity and developing drugs targets in mice.
Postdoc in the Margherita Maffei lab
Dulbecco telethon Institute, Italy
01.2012 - 01.2015
- Establishment, validation, and interpretation of immuno-assays including ELISAs for the detection of leptin and insulin analogues in human serum samples.
PhD Candidate in Enrica Strettoi Lab
Neuroscience Institute of National Research Council, Pisa, Italy
Pisa
01.2009 - 01.2012
- Project management of multiple concurrent projects to achieve milestones.
- Writing papers and reviews for per review scientific journals.
- Leading international collaborations.
Education
Bachelor of Science - Biotechnology
Catholic University of Rome
Rome, Italy04.2006
Master of Science - Neuroscience
La Sapienza University of Rome
Rome, Italy07.2008
Ph.D. - Neuroscience
University of Pisa
Pisa, Italy04.2012
Timeline
Associated Scientist
Boston Children's Hospital
08.2024 - Current
Principal Investigator-Senior Scientist
Hemera srl-University of Milan and Verona Spin-off
01.2023 - Current
Adjunct Faculty Part Time
Eastern Virginia Medical School- Department of Reproductive Clinical Science
05.2022 - 04.2023
Teaching Fellow for the GenED course 1038 Sleep
Harvard Medical School - Harvard teaching hospital
01.2020 - 01.2023
Postdoctoral Research Fellow in Lipton Lab
Harvard Medical School - Boston Children’s Hospital, USA
01.2017 - 01.2023
Postdoc in the Margherita Maffei lab
Scuola Normale Superiore, Pisa, Italy
01.2015 - 01.2016
Postdoc in the Margherita Maffei lab
Dulbecco telethon Institute, Italy
01.2012 - 01.2015
PhD Candidate in Enrica Strettoi Lab
Neuroscience Institute of National Research Council, Pisa, Italy
01.2009 - 01.2012
Bachelor of Science - Biotechnology
Catholic University of Rome
Master of Science - Neuroscience
La Sapienza University of Rome
Ph.D. - Neuroscience
University of Pisa
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