Manuela Saccente

Police officer with almost ten years of experience. I am responsible for coordinating subordinates.
Forensic genetic analyst.
In my work I mainly deal with criminalistics, starting from the inspection of finds from crime scenes, to the sampling of stains, up to the interpretation of the results (electropherograms), with possible biostatistical calculation on single and mixed gen profiles.

1. Registered in the Special List of the Register of Biologists - Section A, with Num . registration: EA_018894

2. Since 2017 - Member of the ISFG - International Society for Forensic Genetics (member 3336)

3. Since 2017 - Registered with Ge.FI - Italian Forensic Geneticists

4. Course "General health and safety training at work for workers and their equivalents" organized by Sapienza University of Rome - March 2024

5. Course for the use of the Crime-lite ML2 Benchtop instrument Laboratory Light Source, organized by Foster+Freeman – 22 February 2023

6. Diploma of the 43rd Civil Military Cooperation Course ( Co.Ci.M. ) - Academic Year 2021/2022, held at the Center for Higher Defense Studies in Rome from 7 to 25 November 2022

7. "Uncertainty and Research ” course , organized by Johns Hopkins University, present on the Coursera platform - May 2022

8. Course "Forensic genetics in civil and criminal fields" organized by the National Academy of Medicine - March 2022

9. 32-hour course in “Scientific communication through the publication of articles and the organization of events. Second edition” organized by the Higher Institute of Health - 2022

10. Diploma of attendance and passing of the final exam of the 20th CBRN basic operational qualification course for Managers, Senior Officers and Functionaries (OP1.3), held at the Joint Forces School for Biological and Chemical Nuclear Defense in Rieti from 8 November to November 12, 2021

11. Participation and passing of the final exam of the Course for Lead Auditors of Quality Management Systems (UNI EN ISO 9001:2015), organized by the Italian Association of Quality Culture (AICQ), Rome - 2021

12. Course “UNI CEI EN ISO/IEC 17025:2018: general requirements for the competence of Testing and Calibration Laboratories”, organized by the National Order of Biologists - 2021

13. “Course on the foundations of statistics and the calculation of measurement uncertainty”, organized by the “Bicocca” University of Milan - 2020

14. Thermo Fisher “Applied Biosystems Rapid HIT ID Install Training” course - 2019

15. Course "The new UNI CEI EN ISO/IEC 17025:2018 standard: focus on the new requirements of Quality Management Systems for Testing and Calibration Laboratories", organized by the National Association for Quality Assurance (ANGQ) - 2019

16. 8th Cycle of the Qualification Course in Investigative and Technical-Scientific Techniques, reserved for State Police officials, at the Higher Police School - Rome - 2017

17. Course on Qiagen Investigator 24 plex kits and other Qiagen solutions for forensic genetics applications - 2017

18. Training course “Quality Management Systems for Testing Laboratories according to ISO/IEC 17025 and Accredia accreditation. Application of forensic genetic evidence” - 2017

19. Training course “Calculation of measurement uncertainty in quantitative and qualitative tests. Validation of the forensic genetics test method - 2017

20. Training course “ISO 9001:2015 requirements: practical application tools” – 2017

Teaching activities/speaker/assignments:

1. International mission organized by CEPOL - European Union Agency for Law Enforcement Training - CEPOL Course 86/2022 " Analysis of complex DNA profile ", held from 17 to 21 October 2022 in Riga (Latvia);

2. Expert speaker in Forensic Genetics at the International Forum on Traces and Corpses entitled "New strategies and new protocols at the crime scene", held at the University of Foggia, on 06 and 07 May 2022;

3. Member of the Working Group established within the Scientific Police Service for the study concerning the analysis of mixed genetic profiles and for the biostatistical calculation on the same - 2019;

4. Member of the Working Group established within the Scientific Police Service for the study concerning the biostatistical calculation on mixed genetic profiles - 2019;

5. Teaching at the DAC (Central Anti-Crime Directorate) qualification courses in investigative and technical-scientific techniques reserved for operators in service at Mobile Squads, Scientific Police Cabinets, DIGOS, PG Teams of the Road and Railway Police Compartments, at the Institute of training of the Nettuno State Police (2018, 2019 and 2020);

6. Speaker at the Career day “What will I do when I grow up? Job opportunities for a biologist”, held at the Department of Bioscience and Territory of the University of Molise - 2018;

7. Teaching for the 1st training course for Deputy Commissioners of the State Police, at the Higher Police School - 2017.

1. Virtual participation at the ISFG Summer School workshop entitled “ Biogeographical ancestry analyzes - Basic” organized by the International Society of Forensic Genetics (ISFG), on 28 August 2023;

2. Virtual participation at the ISFG Summer School workshop entitled “ Inference of relationships - Basic kinship statistics ” organized by the International Society of Forensic Genetics (ISFG), on 28 August 2023;

3. Virtual participation at the ISFG Summer School workshop entitled “ Inference of relationships - Advanced kinship statistics ” organized by the International Society of Forensic Genetics (ISFG), on 29 August 2023;

4. Virtual participation at the ISFG Summer School workshop entitled “Pedigree analysis - Basic” organized by the International Society of Forensic Genetics (ISFG), on 30 August 2023;

5. Virtual participation at the ISFG Summer School workshop entitled “Pedigree analysis - Advanced” organized by the International Society of Forensic Genetics (ISFG), on 31 August 2023;

6. Virtual participation at the ISFG Summer School workshop entitled “Evaluation of biology results given activity level propositions using Bayesian networks - Basic networks: Basic” organized by the International Society of Forensic Genetics (ISFG), on 31 August 2023;

7. Virtual participation at the ISFG Summer School workshop entitled " Bayesian networks and activity level reporting - Advanced" organized by the International Society of Forensic Genetics (ISFG), on 1 September 2023;

8. Virtual participation at the ISFG Summer School workshop entitled “An introduction to and use of microhaplotypes in forensics ” organized by the International Society of Forensic Genetics (ISFG), on 1 September 2023;

9. Virtual participation at the ISFG Summer School workshop entitled “ Y- chromosome interpretation - extended ” organized by the International Society of Forensic Genetics (ISFG), on 4 September 2023;

10.Virtual participation at the  ISFG Summer School workshop entitled “ Y- chromosome interpretation - standard Y- STRs and the use of YHRD” organized by the International Society of Forensic Genetics (ISFG), on 5 September 2023;

11.Virtual participation at the ISFG Summer School workshop entitled “ Mixture interpretation - Basic use of the software EuroForMix and DNAStatistX ” organized by the International Society of Forensic Genetics (ISFG), on 4 September 2023;

12.Virtual participation at the ISFG Summer School workshop entitled “ Mixture Advanced interpretation . Using EuroForMix , DNAStatistX and accompanying software” organized by the International Society of Forensic Genetics (ISFG), on 5 September 2023;

13.Virtual participation at the ISFG Summer School workshop entitled “ Body fluid identification with main focus on RNA” organized by the International Society of Forensic Genetics (ISFG), on 8 September 2023;

14. Ge.FI Workshop in “ Quality certification of scientific data in the courtroom”, held at the University of Messina, 27 April 2023, organized by Ge.FI. (Italian Forensic Geneticists);

15. 45th SIMLA National Congress (Italian Society of Forensic Medicine and Insurance “Forensic Medicine between Law and Rights”, held in Bari, from 26 to 28 May 2022, organized by SIMLA;

16.Participation in the International Forum on Traces and Corpses entitled "New strategies and new protocols at the crime scene", held at the University of Foggia, on 06 and 07 May 2022;

17.Webinar “ Forensic Genetics Genealogy – Validation to Identification and Everything in Between ” organized by Qiagen - 2022;

18.Webinar “Using Forensic Genetics Genealogy – A Practical Guide for Law Enforcement” organized by Verogen - 2022;

19.Webinar “DNA Mixtures: A NIST Scientific Foundation Review” organized from NIST (National Institute of Standards and Technology) - 2021;

20.Webinar "Forensic Genetics: from the study of chromosomal polymorphisms to Molecular Biology Techniques", organized by the Delegation of the National Order of Biologists of Puglia and Basilicata and the Tor Vergata University - 2021;

21.Workshop " Privacy and particular data - The new EU regulation GDPR 679/16", organized by GeFI in collaboration with SIMLA and ONB - 2021;

22.Attendance of the online workshop "DNA phenotyping for forensic purposes - How far can science go", organized by GeFI in collaboration with SIMLA and ONB , on 06 May 2021;

23.Online workshop "National DNA Database - The role of laboratories in its implementation", organized by GeFI in collaboration with SIMLA and ONB - 2021;

24.Webinar “Stability and Persistence of Touch DNA for Forensic Analysis”, organized by Forensic Technology Center of Excellence and National Institute of Justice USA - 2021;

25.Webinar “ Implementing a Y-Screening/Direct to DNA Workflow”, organized by Promega - 2021;

26.Workshop “ Implementing Rapid DNA with confidence”, organized by Thermo Fisher Scientific - 2021;

27.Webinar “Emerging Methods for Body Fluid Analysis”, organized by Forensic Technology Center of Excellence and National Institute of Justice USA - 2021;

28.Biostatistics course “DNA Mixtures Analysis and statistical interpretation ”, organized by ENFSI - 2020;

29.Webinar “An Automated Single Cell Separation Technique to Improve Mixtures Deconvolution ”, organized by Forensic Technology Center of Excellence and National Institute of Justice USA - 2020;

30.Webinar “Validation and Tutorial of NOCIt for Determining the Number of Contributors”, organized by Forensic Technology Center of Excellence and National Institute of Justice USA - 2020;

31.Webinar “Development and Publication of New Standards and Best Practices – The Process”, organized by Promega, OSAC and ASB - 2020;

32.International online workshop “ Uncertainty in Forensic Science” – as part of the Exchange Project Symposium organized by the European Research Council and hosted at the Center for Research on Communication and Society (CECS), entitled “Challenges to criminal justice and police and judicial cooperation : the DNA data exchange within the Prum System” - 2020;

33.Webinar “From Collection to Prosecution: Investigations using Rapid DNA and Local DNA Database”, organized by Thermo Fisher Scientific and Bode Technology - 2020;

34.Webinar “Investigative Genetics Genealogy and Solving Violent Crime”, organized by Bode Technology - 2020;

35.Webinar “Post Conviction DNA Testing – A Crime Lab's Perspective ”, organized by Bode Technology - 2020;

36.Webinar “ Implementation of Rapid DNA in Law Enforcement”, organized by Thermo Fisher Scientific and Bode Technology - 2020;

37.“ DNAxs ” online course, organized by the Dutch Forensic Institute of the Dutch Ministry of Justice and Security ( Ministry of Justice and Security - Netherlands ForensicInstitute ) - 2020.

38.Course in Forensic Genetics “Applications of Next Generation Sequencing Protocols in Forensic Genetics: practical execution and interpretation”, held at the University of Rome Tor Vergata - 2019;

39.XXVIII Congress of the International Society of Forensic Genetics (ISFG), held in Prague (Czech Republic) - 2019;

40.“14th European CODIS” Conference, held in Rome - 2018;

41.Conference “54th EDNAP meeting”, held in Rome - 2018;

42.Conference “42nd ENFSI DNA EXPERT WORKING GROUP meeting”, held in Rome - 2018;

43.Participation in the ISFG Summer School in Forensic Genetics “DNA interpretation in criminal casework using probabilistic software”, held in Catanzaro - 2018;

44.Participation in the “XXVII Ge.FI CONGRESS” congress . DNA AND TECHNOLOGY: two tools to fight crime”, held in Catanzaro – 2018;

45.Participation in the “Tech Day” course Scientific Police - Technical focus: forensic sample collection , organized by COPAN - 2018;

46.Participation in the " Contamination Risk and Prevention " course, organized by Thermo Fisher Scientific - 2018;

47.Quantifiler Trio DNA quantification kit and the Global Filer PCR and Yfiler PCR amplification kits - 2017;

48.Participation in the HIDS Conference – Human IDentification Solutions, organized by Thermo Fisher Scientific, held in Vienna (Austria) - 2017;

49.Participation in the conference “DNA Evidence to Investigative Insights Conference” organized by the Illumina company - 2017;

50.Participation in the event “The establishment of the national DNA database: what is changing in Italy” – 2016.

Good knowledge of the Office package and navigation programs

Knowledge and use of biostatistics software (e.g. LRmix Studio, EuroForMix, Familias, DNAxs, STRmix).

Dr. Alessandra La Rosa : First Technical Director of the State Police –

Director of Division V - Scientific Police Service - Central Directorate for Scientific Police and Cyber Security - Ministry of the Interior (e-mail: alessandra.larosa@interno.it )