Overview
Work History
Education
Skills
Dissemination and Outreach Activities
Timeline
Generic

Viviana Perrotta

Marano di Napoli

Overview

1
1
year of professional experience

Work History

Research Fellow

IBB-CNR
05.2024 - Current

Institute of Biostructures and Bioimaging, IBB‑CNR (National Research Council), Italy


Project (funded by the European Union’s NextGenerationEU):

ENIGMA‑TB – “Understanding PE_PGRS, vital cell‑wall proteins promoting Mycobacterial survival in TB.”

The aim of this project is the structural and functional characterization of a selected panel of PE-PGRS proteins to better understand the mechanisms involved in the pathogenesis of Mycobacterium tuberculosis.

Education

University of Naples "Federico II"
Italy
10-2023

Skills

Hands-on skills

  • Recombinant protein expression and purification:

expertise in heterologous expression systems and downstream purification techniques

  • Molecular biology:

expertise in cloning methods and in analyzing DNA and proteins via electrophoresis (agarose gels, SDS‑PAGE)

  • Structural and functional protein characterization:

protein crystallization for structural studies; circular dichroism (CD) spectroscopy to assess protein folding and secondary structure; light-scattering analyses (Dynamic Light Scattering, SEC‑LS) to determine protein size and aggregation; fluorescence-based assays and investigating protein–ligand interactions through isothermal titration calorimetry (ITC)

Languages

  • English: B2 certified

Technical Skills

  • Proficient in Microsoft Office Suite (Word, Excel, PowerPoint)
  • Experienced with OriginLab for data analysis and graph creation

Dissemination and Outreach Activities


  • Science communication activities within the S.T.R.E.E.T.S Project (Science Technology Research for Ethical Engagement Translated in Society).
  • Public outreach contribution during the 38th Edition of Futuro Remoto – CO-SCIENZE science festival.
  • Poster presentation at the 18th Naples Workshop on Bioactive Peptides – Peptides in the Nativity . Title: “Understanding PE_PGRS: Cell wall proteins promoting mycobacterial survival in TB.”
  • Flash presentation at the 6th BMM–AIC Meeting, Fiesole (FI). Title: “Understanding PE-PGRS: Cell wall proteins promoting mycobacterial survival in TB.”

Timeline

Research Fellow

IBB-CNR
05.2024 - Current

University of Naples "Federico II"
Viviana Perrotta